文章基本信息

标题：False Positive Blood Tacrolimus Concentration in Microparticle Enzyme Immunoassay
本地全文：下载
作者：Masato Homma ; Takashi Tomita ; Kenji Yuzawa 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2002
卷号：25
期号：8
页码：1119-1120
DOI：10.1248/bpb.25.1119
出版社：The Pharmaceutical Society of Japan
摘要：The difference in the blood concentration of tacrolimus between the assay methods, microparticle enzyme immunoassay (MEIA) and enzyme linked immunosorbent assay (ELISA) was observed in a liver transplant recipient with anemia. MEIA provided significantly higher concentration than those of ELISA (7.8±1.9 vs. 5.0±1.8 ng/ml, p <0.05) while the patient had low haematocrit <25%. The difference, however, was not observed during the periods with haematocrit >25%. This observation suggested that unknown tacrolimus levels generated from difference in assay methods gave incorrect blood tacrolimus during anemia. False positive concentration of tacrolimus ranging 0.1—3.3 ng/ml was observed in MEIA applying to the blood samples obtained from the patients without receiving tacrolimus. The false positive tacrolimus increased in the samples with lower hematocrit, suggesting that MEIA gave incorrect blood tacrolimus during anemia. Since MEIA potentially overestimates the tacrolimus levels, ELISA should be used for blood tacrolimus monitoring in the patients with anemia.
关键词：false positive;tacrolimus;microparticle enzyme immunoassay;anemia