文章基本信息

标题：Stimulative Effects of (22E,24R)-Ergosta-7,22-diene-3β,5α,6β-triol from Fruiting Bodies of Tricholoma auratum, on a Mouse Osteoblastic Cell Line, MC3T3-E1
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作者：Keishi Hata ; Fuyuki Sugawara ; Naganori Ohisa 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2002
卷号：25
期号：8
页码：1040-1044
DOI：10.1248/bpb.25.1040
出版社：The Pharmaceutical Society of Japan
摘要：We screened the differentiation-inducing activities of 39 mushroom extracts from Akita prefecture, Japan, on the mouse osteoblastic cell line, MC3T3-E1. Sixteen phosphate buffered saline (PBS), 8 boiled PBS, 14 ethanol and 12 methanol extracts induced alkaline phosphatase (ALP) activities, an indicator of MC3T3-E1 cell differentiation. The enzyme activities were markedly induced by extracts of Tricholoma auratum , and we isolated the active compound from methanol extracts of this mushroom. Physical data for the isolated active compound were identical to those for (22 E ,24 R )-ergosta-7,22-diene-3β,5α,6β-triol (1). 1 induced ALP activities of MC3T3-E1 cells and promoted cell proliferation. To investigate the relationships between the chemical structure and differentiation-inducing activity of the compound, ALP-inducing activities of MC3T3-E1 cells by 1, ergosterol (2), ergocalciferol (3), cholesta-3β,5α,6β-triol (4), 7-dehydrocholesterol (5) and cholecalciferol (6) were tested. The enzyme activities of MC3T3-E1 cells were increased 3.0-fold by 10 μ M 1 and 2.4-fold by 10 μ M 4. However, 2, 3, 5 and 6 did not induce MC3T3-E1 cell ALP activity at 0.1—10 μ M . These results suggested that the OH groups at C-5 and/or C-6 of 1 and 4 played an important role in their differentiation-inducing activities on MC3T3-E1 cells. Furthermore, 1 suppressed induction of MC3T3-E1 cell apoptosis by serum starvation.
关键词：MC3T3-E1 cell;differentiation;anti-osteoporosis;mushroom;Tricholoma auratum;(22E,24R)-ergosta-7,22-diene-3β,5α,6β-triol