文章基本信息

标题：Lumin, a Cyanine Dye, Enhances Interleukin 12-Dependent Interferon Gamma Production by Lipopolysaccharide-Stimulated Mouse Splenocytes
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作者：Toshio Kunikata ; Tatsuya Ishihara ; Shimpei Ushio 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2002
卷号：25
期号：8
页码：1018-1021
DOI：10.1248/bpb.25.1018
出版社：The Pharmaceutical Society of Japan
摘要：Lumin was orally administered to mice daily for 3 d, and on the day following the final administration, mice were sacrificed and splenocytes were stimulated with lipopolysaccharide (LPS). Splenocytes obtained from lumin-treated mice showed enhanced production of interferon γ (IFN-γ) and increased percentages of CD3⁺ cells. Although T cells are considered to be the source of IFN-γ, it is unlikely that LPS directly stimulates T cells. Next we performed neutralization experiments using a monoclonal antibody (mAb) against interleukin (IL-)12 because this cytokine, which is produced by macrophages, has the direct ability to induce IFN-γ production and the proliferation of activated T cells. This antibody inhibited IFN-γ production by splenocytes. We thus show that orally administered lumin enhances IFN-γ production by splenocytes when the latter are stimulated with LPS, a phenomenon that was observed in correlation with activation of T cells by IL-12, that is produced by macrophages.
关键词：photosensitizer;lumin;interferon γ (IFN-γ);lipopolysaccharide (LPS)