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标题：Effect of SCG, 1,3-β-D-Glucan from Sparassis crispa on the Hematopoietic Response in Cyclophosphamide Induced Leukopenic Mice
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作者：Toshie Harada ; Noriko Miura ; Yoshiyuki Adachi 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2002
卷号：25
期号：7
页码：931-939
DOI：10.1248/bpb.25.931
出版社：The Pharmaceutical Society of Japan
摘要：Sparassis crispa Fr. is an edible mushroom recently cultivable in Japan. It contains a remarkably high content of 6-branched 1,3-β- D -glucan showing antitumor activity. Using ion-exchange chromatography, a purified β-glucan preparation, SCG, was prepared. In this study, we examined the hematopoietic response by SCG in cyclophosphamide (CY)-induced leukopenic mice. SCG enhanced the hematopoietic response in CY induced leukopenic mice by intraperitoneal routes over a wide range of concentrations. SCG enhanced the hematopoietic response in CY-treated mice by prior or post administration. Analyzing the leukocyte population by flow cytometry, monocytes and granulocytes in the peritoneal cavity, liver, spleen and bone marrow (BM) recovered faster than in the control group. The ratio of natural killer cells and γδ T cells in the liver, spleen and peritoneal cavity was also increased. In contrast, CD4⁺ CD8⁺ cells in the thymus were temporarily significantly decreased by the administration of SCG. Interleukin-6 (IL-6) production of CY+SCG-treated peritoneal exdated cells (PECs), spleen cells and bone marrow cells (BMCs) were higher than that of the CY-treated group. By in vitro culture of CY-treated PEC and spleen cells, IL-6 production was enhanced by the addition of SCG. These facts suggested the possibility that IL-6 might be a key cytokine for the enhanced hematopoietic response by SCG.
关键词：SCG;β-glucan;hematopoietic response;interleukin-6;natural killer cell