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标题：Prevention of Growth and Metastasis of Murine Melanoma through Enhanced Natural-Killer Cytotoxicity by Fatty Acid-Conjugate of Protopanaxatriol
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作者：Hideo Hasegawa ; Ryuichi Suzuki ; Takema Nagaoka 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2002
卷号：25
期号：7
页码：861-866
DOI：10.1248/bpb.25.861
出版社：The Pharmaceutical Society of Japan
摘要：Ginsenosides, the glycosides of Panax ginseng , are metabolized (deglycosylated) by intestinal bacteria after oral administration. 20( S )-Protopanaxatriol (M4) is the main bacterial metabolite of protopanaxatriol-type ginsenosides and mediates their antitumor effects. To clarify the mechanism of the M4-mediated antitumor effect, the antitumor activity and metabolism of M4 was examined, using the C57BL/6 mice implanted with B16-BL6 melanoma. The chronic oral administration of M4 inhibited the growth of B16-BL6 melanoma at the implanted site. Analyses using TLC, HPLC, MS and NMR suggest that orally administered M4 was absorbed from the small intestine into the mesenteric lymphatics followed by the rapid esterification of M4 with fatty acids and its accumulation in the tissues including the liver and lung. The administration of M4 prior to the intravenous injection of B16-BL6 cells abrogated the enhanced lung metastasis in the mice pretreated with 2-chloroadenosine more effectively than in those pretreated with anti-asialo GM1. The esterified M4 (EM4) did not directly affect tumor growth in vitro , whereas it stimulated splenic NK cells to become cytotoxic to tumor cells. These results indicate that the antitumor activity of M4 is based on the NK cell-mediated tumor lysis enhanced by EM4.
关键词：ginsenoside;20(S)-protopanaxatriol (M4);fatty acid;esterification;immunostimulation;NK cell