文章基本信息

标题：A Novel Protein with Alkaline Phosphatase and Protease Inhibitor Activities in Streptomyces hiroshimensis
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作者：Masayuki Nitta ; Mariko Goto ; Naomi Shibuya 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2002
卷号：25
期号：7
页码：833-836
DOI：10.1248/bpb.25.833
出版社：The Pharmaceutical Society of Japan
摘要：A novel alkaline phosphatase (S-ALP) was found in the culture filtrate of Streptomyces hiroshimensis IFO 12785. Purification was achieved on Sephadex G-75 column, palmitoylated gauze column, and Superdex 75 HR column chromatographies. The molecular weight of S-ALP was estimated to be 14200 by sodium dodecyl sulfate- polyacrylamide gel electrophoresis (SDS-PAGE). The isoelectric point is 9.2. S-ALP had maximum enzyme activity at pH 9.5. S-ALP efficiently catalyzed both p -nitrophenyl phosphate and p -nitrophenyl phosphorylcholine substrates, particularly the latter. The N-terminal amino acid sequence (25 residues) of S-ALP was 60 to 72% identical to that of Streptomyces subtilisin inhibitor-like proteins. S-ALP exhibited trypsin inhibition in addition to a strong inhibition of subtilisin.
关键词：Streptomyces hiroshimensis;alkaline phosphatase;protease inhibitor;subtilisin inhibitor;trypsin inhibitor