文章基本信息

标题：cDNA Cloning of the Sry-Related Gene Sox6 from Rat with Tissue-Specific Expression
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作者：Masanori Narahara ; Akihisa Yamada ; Michiko Hamada-Kanazawa 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2002
卷号：25
期号：6
页码：705-709
DOI：10.1248/bpb.25.705
出版社：The Pharmaceutical Society of Japan
摘要：A cDNA encoding rat homologue of the previously characterized mouse Sox6 was isolated by a polymerase chain reaction (PCR) cloning strategy. Comparison of this cDNA with homologous mouse, human and rainbow trout cDNA exhibited an overall amino acid sequence identity of 99.6, 89.3 and 76.3% respectively. The leucine-zipper and HMG-box motif were almost completely conserved between these homologues. The expression of Sox6 was determined in rat by Northern hybridization and Real-time quantitative reverse transcription (RT)-PCR. rSox6 (rat Sox6) was specifically expressed in the neonatal brain and adult testis with Northern blotting. Real-time quantitative RT-PCR for the determination of Sox6 mRNA was examined. The rSox6 was expressed in the neonatal brain and adult testis as well as by Northern blotting and also expressed in the adult eyeball and slightly in the ovary.
关键词：sry;Sox6;polymerase chain reaction (PCR) cloning;Real-time quantitative reverse-transcription (RT)-PCR