文章基本信息

标题：Molecular Cloning, Functional Expression and Characterization of d-Limonene Synthase from Agastache rugosa
本地全文：下载
作者：Takuro Maruyama ; Daisuke Saeki ; Michiho Ito 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2002
卷号：25
期号：5
页码：661-665
DOI：10.1248/bpb.25.661
出版社：The Pharmaceutical Society of Japan
摘要：We cloned the gene of d -limonene synthase (ArLMS) from Agastache rugosa (Labiatae). The function of ArLMS was elucidated by the preparation of recombinant protein and subsequent enzyme assay. ArLMS consisted of 2077 nucleotides including 1839 bp of coding sequence that encodes a protein of 613 amino acids. This protein has a 60 kDa molecular weight, which is identical to that of d -limonene synthase from Schizonepeta tenuifolia (Labiatae). The deduced amino acid sequence of ArLMS shows high homology with the known d - and l -limonene synthases from Labiatae plants. Here, we discussed the amino acid residues responsible for the stereochemical regulation in limonene biosynthesis.
关键词：d-limonene synthase;monoterpene biosynthesis;Agastache rugosa (Labiatae);cDNA cloning