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  • 标题:Effects of Adenine-Nucleotides on the Sequence-Specificity of Origin Recognition Complex-Binding to DNA
  • 本地全文:下载
  • 作者:Hitomi Takenaka ; Naoko Takahashi ; Jong-Ryul Lee
  • 期刊名称:Biological and Pharmaceutical Bulletin
  • 印刷版ISSN:0918-6158
  • 电子版ISSN:1347-5215
  • 出版年度:2002
  • 卷号:25
  • 期号:5
  • 页码:652-655
  • DOI:10.1248/bpb.25.652
  • 出版社:The Pharmaceutical Society of Japan
  • 摘要:We examined effects of adenine-nucleotides on the sequence-specificity of origin recognition complex (ORC)-binding to DNA, using a gel electrophoretic mobility shift assay. The sequence-specific DNA binding of ORC was observed in the presence of ATP or ATP-γ-S but not in the presence of adenosine 5′-diphosphate (ADP) or in the absence of any adenine-nucleotides. In contrast, the sequence-independent DNA binding of ORC was observed under any one of these conditions. These results suggest that ATP increases the sequence-specificity of ORC-binding to DNA. In relation to the requirement for incubation at high temperature and inhibition by cardiolipin, there was no significant difference between the sequence-specific and the sequence-independent DNA binding activities of ORC.
  • 关键词:ORC;adenine-nucleotide;DNA replication;origin DNA
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