文章基本信息

标题：Enhancement of Cellular Adenosine Triphosphate Levels in PC12 Cells by Extracellular Adenosine
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作者：Hiroyuki Fujimori ; Masahiro Yasuda ; Hidemitsu Pan-Hou 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2002
卷号：25
期号：3
页码：307-311
DOI：10.1248/bpb.25.307
出版社：The Pharmaceutical Society of Japan
摘要：To elucidate the biological significance of extracellular adenine compounds, the effects of adenosine (Ado) on cellular levels of adenine compounds, especially adenosine triphosphate (ATP), in PC12 cells were studied. Ado and inosine but not adenosine 5′-monophosphate, adenosine 5′-diphosphate, ATP, guanosine, cytosine, thymidine, and uridine, significantly enhanced cellular ATP levels in PC12 cells in time- and dose-dependent manners. Various P1 receptor agonists of Ado did not enhance the ATP level. In addition, theophylline, an antagonist of P1 receptors, did not inhibit the Ado-evoked ATP enhancement. These results suggest that the Ado receptor is not involved in the augmentation of the cellular ATP level induced by Ado in PC12 cells. The ATP-enhancing effect of Ado was potentiated by dipyridamole, an inhibitor of Ado uptake, or coformycin, an inhibitor of Ado deaminase. The effect of Ado on the ATP level was also observed when PC12 cells were incubated in glucose-free medium. Together these results suggest that enhancement of cellular ATP levels in PC12 cells by extracellular Ado might be acceleration of ATP synthesis through the Ado salvage system using hypoxanthine-guanine phosphoribosyltransferase rather than Ado kinase since 5′-iodotubercidin, an inhibitor of Ado kinase, had no effect on the enhancement elicited by Ado.
关键词：PC12 cell;adenosine;ATP synthesis;salvage pathway;P1 receptor