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标题：Purification and Characterization of Mouse Mevalonate Pyrophosphate Decarboxylase
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作者：Akihiro Michihara ; Kenji Akasaki ; Yukio Yamori 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2002
卷号：25
期号：3
页码：302-306
DOI：10.1248/bpb.25.302
出版社：The Pharmaceutical Society of Japan
摘要：Mevalonate pyrophosphate decarboxylase (MPD) in mouse liver was purified by affinity chromatography. The purified enzyme was a homodimer of 46-kDa subunits and had an isoelectric point of 5.0. Kinetic analysis revealed an apparent K _m value of 10 μ M for mevalonate pyrophosphate. The enzyme required ATP as a phosphate acceptor and Mg as a divalent cation, which could be substituted with Mn or Co. Its optimum pH was 4.0—7.0. A comparison with MPD from various other sources revealed the mouse MPD to have essentially the same properties as rat MPD, expect for the optimum pH range. An excess of rabbit anti-rat MPD antibody deleted approximately 80% of the MPD activity in the crude extract of mouse liver. These results suggested that the homodimer of 46-kDa subunits represents the major active form of MPD in mice.
关键词：mevalonate pyrophosphate decarboxylase;purification;characterization;mouse;liver