文章基本信息

标题：Application of Green Fluorescent Protein to Affinity Electrophoresis; Affinity of IgG-Binding Domain C from Streptococcal Protein G to Mouse IgG1
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作者：Hitoshi Kazama ; Keiko Yamada ; Takashi Aoki 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2002
卷号：25
期号：2
页码：168-171
DOI：10.1248/bpb.25.168
出版社：The Pharmaceutical Society of Japan
摘要：Affinity electrophoresis (AEP) using green fluorescent protein (GFP) was studied. We constructed a fusion protein that linked S147PGFP and IgG binding domain C from streptococcal protein G (GFP–SpGC). The affinity of GFP–SpGC for mouse IgG1 was measured. The AEP using GFP does not require a staining step after electrophoresis, and was successful with a non-purified sample. Therefore, this method is simple and useful for measuring many samples such as those used in mutational studies.
关键词：affinity electrophoresis;green fluorescent protein;streptococcal protein G;immunoglobulin G