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标题：Magnolol Has the Ability to Induce Apoptosis in Tumor Cells
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作者：Koji Ikeda ; Hisamitsu Nagase
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2002
卷号：25
期号：12
页码：1546-1549
DOI：10.1248/bpb.25.1546
出版社：The Pharmaceutical Society of Japan
摘要：We previously found that multiple intraperitoneal administration of magnolol from Magnolia obovata inhibited tumor metastasis and growth in vivo , and that the anti-metastatic effect of magnolol was due to the inhibition of the tumor cell invasion. The purpose of this study was to clarify the inhibitory mechanism of magnolol on the growth of tumor cells, and we expect that magnolol may have the ability to induce apoptosis in tumor cells. In an in vitro proliferation assay, 100 μ M of magnolol inhibited the proliferation of B16-BL6, THP-1, BAE and HT-1080 cells, but 30 μ M of magnolol did not affected cells proliferation. In addition, 100 μ M of magnolol induced apoptotic cell death within 24 h in three tumor cell lines, B16-BL6, THP-1 and HT-1080, not BAE cells, and then up-regulated the activity of caspase-3 and caspase-8. The up-regulation of caspases activity by 100 μ M of magnolol was suppressed by the inhibitor of all caspases, z-VAD-fmk. These data suggest that magnolol possesses ability to inhibit tumor growth, and the ability is due to the induction of apoptosis with the activation of caspases.
关键词：magnolol;apoptosis;antitumor effect;caspase;mouse