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标题：Protective Effect of Moutan Cortex Extract on Acetaminophen-Induced Cytotoxicity in Human Chang Liver Cells
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作者：Yun-Hee Shon ; Kyung-Soo Nam
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2002
卷号：25
期号：11
页码：1427-1431
DOI：10.1248/bpb.25.1427
出版社：The Pharmaceutical Society of Japan
摘要：The aim of this study was to investigate the effect of Moutan Cortex on acetaminophen (AAP)-induced toxicity in human Chang liver cells. Cells were incubated with AAP (0—30 m M ) to evaluate the drug’s ability to reduce cytoviability. For the cells treated with 10, 20 and 30 m M AAP, LDH leakage was 39.8%, 49.0% and 57.6%, respectively. Administration of Moutan Cortex reduced cytotoxicity in a dose-dependent manner. Glutathione (GSH) concentration in human liver cells decreased significantly after exposure to 20 ( p <0.05) and 30 m M ( p <0.01) AAP, and increased ( p <0.05) if incubated with AAP and Moutan Cortex. The ability of AAP to inhibit mitochondrial function and its counteraction by Moutan Cortex was also evaluated. Moutan Cortex showed dose-dependent increases in MTT metabolism and ATP levels in AAP-treated cells. The DNA content of AAP-treated cells increased with the treatment of Moutan Cortex. These observations demonstrate that Moutan Cortex may significantly attenuate AAP-induced toxicity. It can be considered a cytoprotective agent in this in vitro model of drug toxicity.
关键词：acetaminophen;Moutan Cortex;glutathione;cytotoxicity;mitochondria;DNA