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标题：Spleen Lymphocyte Kinetics in Mice under Normal and Inflammatory Conditions: An Application of the Transgenic Mouse Expressing β-Galactosidase (ROSA 26)
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作者：Ken-ichi Hosoya ; Tadayuki Takashima ; Ken-ichi Matsuda 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2002
卷号：25
期号：10
页码：1378-1380
DOI：10.1248/bpb.25.1378
出版社：The Pharmaceutical Society of Japan
摘要：The purpose of this study was to develop a method for the measurement of the cell kinetics of spleen lymphocytes using the ROSA 26 transgenic mouse ubiquitously expressing β-galactosidase (β-gal). Spleen lymphocytes were isolated from ROSA 26 mice and intravenously inoculated into C57BL/6 mice under normal conditions and inflammatory conditions following lipopolysaccharide (LPS) treatment. Spleen lymphocyte accumulation in tissues was determined as a measurement of β-gal activity. Spleen lymphocytes isolated from ROSA 26 mice have β-gal activities of 1.45×10⁻⁴ pg per cell. A good correlation between β-gal activities and cell numbers was obtained ( r ²=0.999) over the range 1×10³ to 1×10⁷ cells, corresponding to 70 fg to 350 pg β-gal activity. Spleen lymphocytes (4×10⁷ cells) were intravenously inoculated into normal mice and subsequently each tissue was isolated and the corresponding β-gal activity measured. Spleen lymphocyte accumulation was relatively high in the spleen and lymph nodes. The accumulated spleen lymphocyte cell number was 1.39×10⁷ cells/g spleen and 5.45×10⁷ cells/g lymph node 1 h and 6 h after inoculation, respectively, and this remained constant up to 24 h. In the lung, lymphocyte accumulation was 3.98×10⁷ cells/g tissue 10 min after inoculation then gradually fell to 7.09×10⁵ cells/g tissue after 24 h. In addition, the femoral muscle following intramuscular injection of LPS showed a high accumulation of spleen lymphocytes, whereas the untreated and contralateral femoral muscle had the same level as the background. In conclusion, spleen lymphocytes isolated from ROSA 26 mice can be used to measure β-gal activity and the sensitivity is relatively high over the 70 fg to 350 pg range. This suggests that cells isolated from the ROSA 26 mouse can be applied to the study of cell kinetics.
关键词：cell kinetic;cell delivery;ROSA 26 mouse;β-galactosidase