文章基本信息

标题：Formation of Active Oxygen Species from Diethylstilbestrol, a Synthetic Estrogen, and Its Metabolite in the Presence of RAW 264.7 Cells
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作者：Taiko Oda ; Motohisa Kaneko
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2002
卷号：25
期号：10
页码：1311-1314
DOI：10.1248/bpb.25.1311
出版社：The Pharmaceutical Society of Japan
摘要：Previously, we reported that (±)-IA but not DES produces O₂⁻ spontaneously in PBS. We are interested in the possibility that these compounds might produce active oxygen species under mild cell culture conditions. On incubation of RAW 264.7 cells with (±)-IA, the signal of 5,5′-dimethyl-1-pyrroline- N -oxide (DMPO)-OH adducts increased but no more than the additive effect. However, stimulation of RAW cells with LPS and INF-γ enhanced the formation of DMPO-OH adducts slightly more than the additive effect, especially when the concentration of (±)-IA increased. In the case of DES, the spectra of DMPO-OH adducts did not increase concentration-dependently in the absence of RAW 264.7 cells, however in their presence, they increased concentration-dependently, especially when these cells were stimulated with LPS and IFN-γ. The results were interpreted to mean that DES would have a higher oxidation potential than (±)-IA, not be oxidized to semiquinoes spontaneously, and therefore not produce DMPO-OH adducts in the absence of RAW cells. In their presence, DES might be easily oxidized to semiquinones by the reaction with O₂⁻ produced from RAW 264.7 cells.
关键词：synthetic estrogens;indenestrol A;active oxygen species;RAW 264.7 cells