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标题：Cloning and Characterization of a cDNA Encoding β-Amyrin Synthase Involved in Glycyrrhizin and Soyasaponin Biosyntheses in Licorice
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作者：Hiroaki HAYASHI ; Pengyu HUANG ; Ara KIRAKOSYAN 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2001
卷号：24
期号：8
页码：912-916
DOI：10.1248/bpb.24.912
出版社：The Pharmaceutical Society of Japan
摘要：An oxidosqualene cyclase cDNA, termed GgbAS1, was isolated from cultured cells of licorice (Glycyrrhiza glabra) by heterologous hybridization with cDNA of Arabidopsis thaliana LUP1 lupeol synthase. The yeast transformed with an expression vector containing the open reading frame of GgbAS1 produced β-amyrin, indicating that GgbAS1 encodes β-amyrin synthase involved in the glycyrrhizin and soyasaponin biosyntheses in licorice. Northern blot analysis showed that the level of β-amyrin synthase mRNA was drastically changed in the cultured licorice cells, whereas the mRNA level of cycloartenol synthase was relatively constant.
关键词：Glycyrrhiza glabra;cDNA cloning;β-amyrin synthase;glycyrrhizin;soyasaponin