文章基本信息

标题：Molecular Authentication of Panax ginseng Species by RAPD Analysis and PCR-RFLP
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作者：Jae-Young UM ; Hwan-Suck CHUNG ; Mi-Sun KIM 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2001
卷号：24
期号：8
页码：872-875
DOI：10.1248/bpb.24.872
出版社：The Pharmaceutical Society of Japan
摘要：In order to develop convenient and reproducible methods for the identification of ginseng drugs at a DNA level, randomly amplified polymorphic DNA (RAPD) and PCR-restriction fragment length polymorphism (PCR-RFLP) analyses were applied within Panax species. To authenticate Panax ginseng among ginseng populations, RAPD analysis was carried out using a 20 mer-random primer. The similarity coefficients among the DNA of ginseng plants analyzed were low, ranging from 0.197 to 0.491. In addition, by using PCR-RFLP analysis, very different fingerprints were obtained within Korean ginseng plants. These results suggest that these methods are able to authenticate the concerned Panax species. Broader application of this approach to authenticate other morphologically similar medicinal materials is rationalized.
关键词：RAPD;PCR-RFLP;Panax species;authentication