文章基本信息

标题：Determination of Urinary Pyrraline by Solid-Phase Extraction and High Performance Liquid Chromatography
本地全文：下载
作者：Kazuhiro YOSHIHARA ; Reiko KIYONAMI ; Yumi SHIMIZU 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2001
卷号：24
期号：8
页码：863-866
DOI：10.1248/bpb.24.863
出版社：The Pharmaceutical Society of Japan
摘要：Pyrraline is one of the advanced glycation end products formed under non-enzymatic and non-oxidative conditions in vivo. In this study, we developed a novel method for determination of urinary pyrraline using solid-phase extraction as a pretreatment procedure prior to determination by high performance liquid chromatography (HPLC). The Oasis^TM HLB solid-phase extraction cartridge was used for pretreatment of urine samples without hydrolysis. The chromatogram obtained clearly revealed the peak for urinary pyrraline owing to prior removal of interfering substances in urine samples. The recovery rate of pyrraline was 97.2±3.3% (n=6). The mean excretion level of urinary pyrraline in healthy control (20—77 years old, n=30) was 1.42±0.65 μmol/mmol creatinine, and the daily variation in the excretion level was considered to be insignificant. We propose the above procedure as a simple, rapid, and accurate method for determination of pyrraline levels in urine.
关键词：advanced glycation end product;pyrraline;HPLC;solid-phase extraction;urine;atmospheric pressure chemical ionization tandem mass spectrometry