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标题：Antitumor β-Glucan from the Cultured Fruit Body of Agaricus blazei
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作者：Naohito OHNO ; Mai FURUKAWA ; Noriko N. MIURA 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2001
卷号：24
期号：7
页码：820-828
DOI：10.1248/bpb.24.820
出版社：The Pharmaceutical Society of Japan
摘要：Agaricus blazei is a medically important mushroom widely eaten and prescribed in Japan. Polysaccharide fractions were prepared from cultured A. blazei by repeated extraction with hot water (AgHWE), cold NaOH (AgCA), and then hot NaOH (AgHA). By chemical, enzymic, and NMR analyses, the primary structures of AgHWE, AgCA, and AgHA were mainly composed of 1,6-β-glucan. Among these fractions, the NaOH extracts showed antitumor activity against the solid form of Sarcoma 180 in ICR mice. To demonstrate the active component in these fractions, several chemical and enzymic treatments were applied. These fractions were found to be i) neutral β-glucan passing DEAE-Sephadex A-25, ii) resistant to periodate oxidation (I/B) and subsequent partial acid hydrolysis (I/B/H), iii) resistant to a 1,3-β-glucanase, zymolyase, before I/B, but sensitive after I/B/H. In addition, after I/B/H treatment of the neutral fraction of AgCAE, a signal around 86 ppm attributable to 1,3-β-glucosidic linkage was detectable in the ¹³C-NMR spectrum. These facts strongly suggest that a highly branched 1,3-β-glucan segment forms the active center of the antitumor activity.
关键词：β-glucan;Agaricus blazei;immunomodulation;antitumor activity