文章基本信息

标题：A Sensitive Assay of Human Blood Platelet Cyclic Nucleotide Phosphodiesterase Activity by HPLC Using Fluorescence Derivatization and Its Application to Assessment of Cyclic Nucleotide Phosphodiesterase Inhibitors
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作者：Yoshihito OHBA ; Kyoko SODA ; Kiyoshi ZAITSU 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2001
卷号：24
期号：5
页码：567-569
DOI：10.1248/bpb.24.567
出版社：The Pharmaceutical Society of Japan
摘要：A selective and sensitive HPLC measurement of 3',5'-cyclic nucleotide phosphodiesterase (PDE) activity in human platelets using (3,4-dimethoxyphenyl)glyoxal (DMPG) as a fluorogenic reagent for guanine and its nucleosides and nucleotides is described. cGMP, a substrate for PDE, and GMP, which was produced by the enzyme reaction, are selectively converted by the reaction with DMPG to the fluorescent derivatives. The derivatives were separated by reversed-phase HPLC. Human platelet PDE activity was measured and the inhibitory effects of several compounds were investigated.
关键词：3',5'-cyclic nucleotide phosphodiesterase;cGMP;platelet human;derivatization fluorescence;HPLC