文章基本信息

标题：Influence of Sugar Chain on Fibrin Affinity of Recombinant t-PA
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作者：Shoichi AOKI ; Norihide SHIMIZU ; Manabu SHIMONISHI 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2001
卷号：24
期号：3
页码：295-298
DOI：10.1248/bpb.24.295
出版社：The Pharmaceutical Society of Japan
摘要：The role of the sugar chain on the fibrin affinity property of tissue plasminogen activator (t-PA) was investigated using two variants of wild type t-PA (WT t-PA I and WT t-PA II) and mutant type t-PA (mt-PA; Gln117 t-PA I and Gln117 t-PA II), whose sugar chains have different structures. In terms of fibrin affinity, Gln117 t-PA was higher than WT t-PA; moreover, Type II was higher than Type I. Bindings mediated via finger domain (F mode) and kringle 2 domain (K2 mode) were distinguished using ε-amino caproic acid (EACA). Consequently, F mode and K2 mode bindings were inhibited by the sugar chains at Asn117 and 184, respectively. These results were assumed to be due to the steric hindrance of the sugar chains.
关键词：tissue plasminogen activator;Gln117 t-PA;variant;ε-amino caproic acid