文章基本信息

标题：Molecular Cloning, Functional Expression and Characterization of (E)-β-Farnesene Synthase from Citrus junos
本地全文：下载
作者：Takuro MARUYAMA ; Michiho ITO ; Gisho HONDA 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2001
卷号：24
期号：10
页码：1171-1175
DOI：10.1248/bpb.24.1171
出版社：The Pharmaceutical Society of Japan
摘要：We cloned the gene of the acyclic sesquiterpene synthase, (E)-β-farnesene synthase (CJFS) from Yuzu (Citrus junos, Rutaceae). The function of CJFS was elucidated by the preparation of recombinant protein and subsequent enzyme assay. CJFS consisted of 1867 nucleotides including 1680 bp of coding sequence encoding a protein of 560 amino acids with a molecular weight of 62 kDa. The deduced amino acid sequence possessed characteristic amino acid residues, such as the DDxxD motif, which are highly conserved among terpene synthases. This is the first report of the cloning of a terpene synthase from a Rutaceous plant. A possible reaction mechanism for terpene biosynthesis is also discussed on the basis of sequence comparison of CJFS with known sesquiterpene synthase genes.
关键词：(E)-β-farnesene synthase;sesquiterpene biosynthesis;Citrus junos(Rutaceae)