文章基本信息

标题：Inhibitory Effects of Ethaverine, a Homologue of Papaverine, on Dopamine Content in PC12 Cells
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作者：Jung Soo SHIN ; Jae Joon LEE ; Youngsoo KIM 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2001
卷号：24
期号：1
页码：103-105
DOI：10.1248/bpb.24.103
出版社：The Pharmaceutical Society of Japan
摘要：The inhibitory effects of ethaverine on dopamine content in PC12 cells were investigated. Ethaverine decreased dopamine content in a concentration-dependent manner in PC12 cells and showed 33.6% inhibition of dopamine content at a concentration of 1.0 μM for 24—48 h. The IC₅₀ value of ethaverine was 1.4 μM. Dopamine content was lowered at 6 h and reached a minimal level at 12 h after exposure to ethaverine at 2.0 μM. The decreased dopamine level was maintained up to 48 h and then recovered to the control level at about 72 h. Tyrosine hydroxylase (TH) was inhibited at 6 h following treatment with ethaverine in PC12 cells and the activity was maintained at a reduced level up to 36 h (12—22% inhibition at 2.0 μM). These results indicate that ethaverine leads to a decrease in dopamine content by inhibition of TH activity.
关键词：ethaverine;dopamine content;tyrosine hydroxylase;PC 12 cells