文章基本信息

标题：Envelope virus-mimetic nanovaccines by hybridizing bioengineered cell membranes with bacterial vesicles
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作者：Mengmeng Zhang ; Lu Wang ; Jinyao Liu 等
期刊名称：iScience
印刷版ISSN：2589-0042
出版年度：2022
卷号：25
期号：6
页码：1-18
DOI：10.1016/j.isci.2022.104490
语种：English
出版社：Elsevier
摘要：SummaryEmerging threats of rapid spread highly lethal infectious diseases highlight the urgent need of vaccine development. Here, we describe the preparation of envelope virus-mimetic nanovaccines by hybridizing bioengineered cell membranes with bacterial vesicles. Membranes acquired from bioengineered cells overexpressing viral antigens are fused with bacterial outer membrane vesicles to develop hybrid nanovesicles. Because of the presence of intact viral antigenic proteins with natural conformation bound to lipid bilayer and pathogen-associated molecular patterns, hybrid nanovesicles can strikingly promote antigen uptake, processing and presentation by dendritic cells. Immunization with envelope virus-mimetic nanovaccines shows significantly enhanced maturation and activation of dendritic cells, which elicit robust humoral and cellular immune responses in mice. By virtue of their artificial characteristic and absence of loaded adjuvants, these biomimetic nanovaccines exhibit favorable biosafety. Our work demonstrates the effectiveness of envelope virus-mimetic nanovaccines to boost antigen-specific immunity and proposes a simple yet versatile platform to prepare antiviral vaccines.Graphical abstractDisplay OmittedHighlights•Nanovaccines were hybridized by bioengineered cell membranes and bacterial vesicles•Nanovaccines possessed intact viral antigens with natural conformation•Nanovaccines promoted antigen uptake, processing, and presentation by dendritic cells•Nanovaccines elicited robust humoral and cellular immune responses in miceBiological sciences; Immunology; Virology; Biotechnology