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标题：Mechanistic elucidation of freezing-induced surface decomposition of aluminum oxyhydroxide adjuvant
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作者：Jiahuan Li ; Ge Yu ; Zhihui Liang 等
期刊名称：iScience
印刷版ISSN：2589-0042
出版年度：2022
卷号：25
期号：6
页码：1-17
DOI：10.1016/j.isci.2022.104456
语种：English
出版社：Elsevier
摘要：SummaryThe freezing-induced aggregation of aluminum-based (Alum) adjuvants has been considered as the most important cause of reduced vaccine potency. However, the intrinsic properties that determine the functionality of Alum after freezing have not been elucidated. In this study, we used engineered aluminum oxyhydroxide nanoparticles (AlOOH NPs) and demonstrated that cryogenic freezing led to the mechanical pressure-mediated reduction of surface hydroxyl. The sugar-based surfactant, octyl glucoside (OG), was demonstrated to shield AlOOH NPs from the freezing-induced loss of hydroxyl content and the aggregation through the reduction of recrystallization-induced mechanical stress. As a result, the antigenic adsorption property of frozen AlOOH NPs could be effectively protected. When hepatitis B surface antigen (HBsAg) was adjuvanted with OG-protected frozen AlOOH NPs in mice, the loss of immunogenicity was inhibited. These findings provide insights into the freezing-induced surface decomposition of Alum and can be translated to design of protectants to improve the stability of vaccines.Graphical abstractDisplay OmittedHighlights•The freezing stress led to the destruction of surface hydroxyl group on AlOOH NPs•Octyl glucoside protected AlOOH NPs from freezing-induced surface decomposition•Octyl glucoside protected vaccines from freezing-induced loss of immunogenicityInorganic materials; Biotechnology; Materials science; Materials chemistry