文章基本信息

标题：Transmission of pLVPK-like virulence plasmid in Klebsiella pneumoniae mediated by an Incl1 conjugative helper plasmid
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作者：Xuemei Yang ; Miaomiao Xie ; Qi Xu 等
期刊名称：iScience
印刷版ISSN：2589-0042
出版年度：2022
卷号：25
期号：6
页码：1-14
DOI：10.1016/j.isci.2022.104428
语种：English
出版社：Elsevier
摘要：SummaryWe previously reported the recovery of five ST11 carbapenem resistant hypervirulentKlebsiella pneumoniae(CR-HvKP) strains that harbored pLVPK-like virulence plasmids, yet molecular mechanisms underlying acquisition of virulence plasmid by ST11K. pneumoniaehave not been characterized. In this study, we showed that virulence plasmids in these CR-HvKP strains could be transferred toEscherichia colistrain EC600 via conjugation. Transmission of the virulence plasmids was found to involve formation of fusion plasmids with an Incl1 type conjugative plasmid and a small ColRNAI plasmid through homologous recombination and by insertion sequences IS26and IS903B. The conjugative fusion event would transform different ST types ofK. pneumoniae, in particular, the clinically prevalent ST11 or ST258 CRKP into CR-HvKP. Clinical factors that promote or suppress the occurrence of this fusion process should be further investigated to devise new approaches to halt such bacterial evolution trends.Graphical abstractDisplay OmittedHighlights•The pLVPK-like virulence plasmid could be transferred toEscherichia colivia conjugation•Transmission of the virulence plasmids was found to involve formation of fusion plasmids•The fusion processes involved homologous recombination or insertion sequences IS26/IS903BMicrobiology; Molecular microbiology; Clinical microbiology.