文章基本信息

标题：Heterologous ChAdOx1-BNT162b2 vaccination in Korean cohort induces robust immune and antibody responses that includes Omicron
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作者：Hye Kyung Lee ; Jinyoung Go ; Heungsup Sung 等
期刊名称：iScience
印刷版ISSN：2589-0042
出版年度：2022
卷号：25
期号：6
页码：1-16
DOI：10.1016/j.isci.2022.104473
语种：English
出版社：Elsevier
摘要：SummaryHeterologous ChAdOx1-BNT162b2 vaccination induces a stronger immune response than BNT162b2-BNT162b2. Here, we investigated the molecular transcriptome, germline allelic variants of immunoglobulin loci, and anti-Omicron antibody levels in 46 office and lab workers from the Republic of Korea following ChAdOx1-BNT162b2 vaccination. Anti-spike-specific IgG antibody levels against the ancestral SARS-CoV-2 strain increased from 70 AU/ml to 14,000 AU/ml to 142,000 AU/ml one, three and seven days following the second vaccination. Titers against VOC, including Omicron, were two-fold to three-fold lower, yet higher than those measured following BNT162b2-BNT162b2 vaccination. RNA-seq of peripheral immune cells demonstrated activation of interferon pathways with increased IGHV clonal transcripts encoding neutralizing antibodies. scRNA-seq revealed enriched B cell and CD4+T cell responses in both ChAdOx1-BNT162b2 and BNT162b2-BNT162b2 recipients, but a stronger clonal expansion of memory B cells with ChAdOx1-BNT162b2. In summary, heterologous ChAdOx1-BNT162b2 provides an innate and adaptive immune response that exceeds homologous BNT162b2 vaccination.Graphical abstractDisplay OmittedHighlights•ChAdOx1/BNT162b2 vaccination response exceeds BNT162b2/BNT162b2 response•Stronger activation of interferon-induced genetic programs with ChAdOx1/BNT162b2•Antibody titers higher following ChAdOx1/BNT162b2 vaccination•Antibodies against Omicron elicited but 2-fold to 3-fold lower than ancestral COVID-19Health sciences; Immunology; Immune response