文章基本信息

标题：Expression of PEI-coated gold nanoparticles carrying exogenous gene in periwinkle mesophyll cells and its practice in huanglongbing research
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作者：Junzhe Zhang ; Wenxue Lei ; Yixuan Meng 等
期刊名称：iScience
印刷版ISSN：2589-0042
出版年度：2022
卷号：25
期号：6
页码：1-18
DOI：10.1016/j.isci.2022.104479
语种：English
出版社：Elsevier
摘要：SummaryHuanglongbing (HLB) is a devastating disease of citrus, which is mostly caused byCandidatusLiberibacter asiaticus (CLas). To realize the specific application of nano-transgenic technology in HLB, AuNPs-PEI (Gold Nanoparticles-Polyethylenimine) was used to carry foreign genes into the leaves of periwinkle (Catharanthus roseus) by infiltration. Here, we demonstrated that NPR1-GFP protein expression was observed from the 12th hour to the 10th day after infiltrating AuNPs-PEI-pNPR1 (Arabidopsis thaliana nonexpressor of pathogenesis-related gene 1)-GFP. Fluorescence of mCherry was observed 6 h after AuNPs-PEI-pNLS (nuclear localization signal sequence)-mCherry infiltration and fluorescence of FAM was observed in the nucleus 4 h after AuNPs-PEI-FAM-siRNANPR1infiltration. In addition, NPR1-GFP expression inCLas-infected periwinkle leaves was significantly higher than that in healthy periwinkle leaves after infiltration. Our work confirmed that the expression of exogenous NPR1-GFP could reduce theCLas titers by promoting the expression ofPR(pathogenesis related) genes andICS(isochorismate synthase) gene.Graphical abstractDisplay OmittedHighlights•AuNPs-PEI-FAM-siRNANPR1entered the nucleus within 4 h after infiltration•AuNPs-PEI-pNLS-mCherry expressed the corresponding protein within 6 h•AuNPs-PEI-pNPR1-GFP continued to express the corresponding protein for 14 days•After AuNPs-PEI-pNPR1-GFP infiltration for 2 days,CLas titer decreased significantlyBiological sciences; Biomaterials; Biotechnology; Materials in biotechnology