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标题：Zinc oxide nanoparticle: An effective antibacterial agent against pathogenic bacterial isolates
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作者：Irfan Ahmad ; Mohammad Y. Alshahrani ; Shadma Wahab 等
期刊名称：Journal of King Saud University - Science
印刷版ISSN：1018-3647
出版年度：2022
卷号：34
期号：5
页码：1-7
DOI：10.1016/j.jksus.2022.102110
语种：English
出版社：Elsevier
摘要：BackgroundZinc oxide nanoparticle (ZnO NP) was investigated to find out the next generation nano antibiotics that can be developed from them to counter pathogenic microorganisms responsible for multi-drug resistance. It has been seen that they have unique physicochemical properties that can be utilized against toxicological and biological properties of microorganisms.MethodsApplication of well diffusion and the procedure of micro broth dilution utilizing Alamar blue were done to evaluate the inhibition zone, minimum bactericidal concentration (MBC), and the minimum inhibitory concentration (MIC). Also, the antibacterial effectiveness of ZnO NP that constitutes the genesis of biofilms was analyzed by crystal violet formation assay. Inhibition of bacteria was ascertained through the percentage of inhibition of bacterial colonies upon treating with ZnO NP.ResultsIn this study, we scrutinized the efficacy of ZnO NP on Gram-negative as well as Gram positive infectious strains. Ultimate inferences demonstrated the consequences that ZnO NP possessed statistically significant antibacterial efficacy with the significant inhibitory zone (16–21 mm), minimum inhibitory concentration (15.625–125 μg/ml) and minimum bactericidal concentration (62.5–250 μg/ml). Quantitative estimation of biofilms formed byStreptococcus pyogenes, SalmonellaandKlebsiella pneumoniaeat MIC × 2 of ZnO NP showed 2.3, 2.83 and 2.72-fold decrease, respectively.ConclusionsThe results imply that ZnO NP can be utilized as substitute antibacterial agents especially against Gram positive bacterial isolates, which can help develop various antibiotic agents in the clinical setup.
关键词：Zinc oxide nanoparticlesAntibacterialBiofilmGram-positive bacteriaGram-negative bacteria