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标题：2,4-Di- tert-butylphenol (2,4-DTBP) purified from Streptomyces sp. KCA1 from Phyllanthus niruri: Isolation, characterization, antibacterial and anticancer properties
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作者：Ayswarya Seenivasan ; Radhakrishnan Manikkam ; Manigundan Kaari 等
期刊名称：Journal of King Saud University - Science
印刷版ISSN：1018-3647
出版年度：2022
卷号：34
期号：5
页码：1-7
DOI：10.1016/j.jksus.2022.102088
语种：English
出版社：Elsevier
摘要：ObjectivesThe present study reports the bioactivity of 2,4-Di-tert-butylphenol (2,4-DTBP) purified from an endophyticStreptomycesspecies KCA1 isolated from the leaves ofPhyllanthus niruriand its antibacterial and anticancer properties.MethodsThe extracellular metabolites were produced from the strain KCA1 by submerged fermentation using ethyl acetate. The crude extract was further evaluated for antibacterial activity against set of pathogens. Active metabolite from the extract was purified using chromatography techniques and detected its antibacterial activity by disc diffusion assay. The molecular structure of the active molecule was identified through various spectral study. Moreover, the bioactive metabolite 2,4-DTBP was analyzed antibacterial and anti-proliferative activity.ResultsStrain KCA1 was identified asStreptomycessp. In the preliminary screening, the crude extract exhibited broad spectrum activity against various bacterial pathogens. Based on the spectral properties, the active metabolite was identified as 2,4-Di-tert-butylphenol. The MIC of active compound 2,4-DTBP inhibitedE. coliATCC 25922 andS. aureusATCC 29213 at 50 μg/ml and 0.78 μg/ml, respectively. The IC50 value of 2,4-DTBP was found to be 11.0 µg/ml and 116.8 µg/ml, against breast cancer cell line (MCF7) and normal VERO cell line, respectively.ConclusionsThis study concluded that 2,4-DTBP, produced from the endophyticStreptomycessp. KCA1, is the potential candidate to develop as promising antibacterial and anticancer agent.
关键词：EndophyticStreptomyces2,4-Di-tert-butylphenolAntibacterialAntiproliferative activity