文章基本信息

标题：Conjugation in the eukaryotic single-celled organism Euplotes aediculatus (Protozoa, Ciliophora): A focus on nuclear divisions, morphogenesis and pheromones
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作者：Ruitao Gong ; Yong Chi ; Chen Shao 等
期刊名称：Journal of King Saud University - Science
印刷版ISSN：1018-3647
出版年度：2022
卷号：34
期号：5
页码：1-8
DOI：10.1016/j.jksus.2022.102091
语种：English
出版社：Elsevier
摘要：Ciliates are unique in having nuclear dimorphism (i.e., possessing both a germline micronucleus and a somatic macronucleus in one cell), and undergo dramatic changes during their sexual process (known as conjugation). Here we investigate the nuclear events and morphogenetic processes during conjugation in the freshwater speciesEuplotes aediculatus. In addition, three conjugation-related pheromone genes were sequenced and analyzed. The results indicate that: (i) the 65-hour-long conjugation process includes five nuclear divisions, i.e., three prezygotic divisions (mitosis, meiosis I and II) and two postzygotic divisions; (ii) the ciliature reorganizes twice during conjugation with the frontoventral-transverse cirral anlagen regenerating during both morphogenetic events and breaking apart in a 3:3:3:2:2 pattern; and (iii) each of three pheromone genes contains a 444 bp coding region and encodes 147 amino acid polypeptides. These results suggest a broadly shared morphogenetic pattern during conjugation inEuplotesspecies and provide new information for studies on the evolutionary history of pheromone genes.
关键词：CiliateConjugationEuplotes aediculatusMorphogenesisNuclear eventsPheromone genes