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标题：Acetylcholinesterase inhibitory activity and iron-induced lipid peroxidation reducing potential of Pimpinella stewartii leaves in male wistar rats
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作者：Muhammad Umar Khayam Sahibzada ; Wasim Ahmad ; Ahmed M. El-Shehawi 等
期刊名称：Journal of King Saud University - Science
印刷版ISSN：1018-3647
出版年度：2022
卷号：34
期号：4
页码：1-5
DOI：10.1016/j.jksus.2022.101993
语种：English
出版社：Elsevier
摘要：AbstractThis study was aimed to determine the potentialities ofPimpinella stewartiileaves extracts fractions towards the inhibition of acetylcholinesterase (AChE) activity as well as reduction of iron-induced lipid peroxidation in male Wistar rats (age – 50 days; weight – 280–300 g). Initially, animals were divided into 17 groups with 7 rats in each group. Gallic acid and quercetin were used as standards. The AChE activities of aqueous and ethanolic extract fractions ofP. stewartiileaves were determined using standard protocol. Results showed that ethyl acetate extract fractions significantly (P = 0.01) inhibited the enzyme in a concentration dependent manner when compared to the aqueous extract fractions and the control group. Gallic acid and quercetin depicted higher inhibition of AChE activities than ethyl acetate extract fractions. Additionally, the IC50values for aqueous extract fractions, ethyl acetate extract fractions, gallic acid, and quercetin were estimated as 72.6 ± 2.72, 30.41 ± 0.56, 8.4 ± 0.22, and 6.82 ± 0.2 µg/mL, respectively. Furthermore, the lipid peroxidation activities of extracts fractions were assessed by estimating thiobarbituric acid reactive substances (TBARS) production. Both the extracts fractions showed significant protection against lipid peroxidation but the ethyl acetate extract fractions exhibited comparatively higher reductions in TBARS levels (P = 0.001). However, gallic acid and quercetin revealed higher reductions of pro-oxidants than the extracts fractions. In conclusion, findings of this study demonstrated pronounced AChE inhibitory activity and reduced lipid peroxidation trait of ethyl acetate extract fractions ofP. stewartiileaves, and thus, suggested its paramount role as therapeutics against Alzheimer disease.
关键词：KeywordsenAcetylcholinesterase activityAlzheimer diseaseLipid peroxidationPimpinella stewartiileavesTherapeutics