文章基本信息

标题：Non-destructive mechanical assessment for optimization of 3D bioprinted soft tissue scaffolds
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作者：Brent Godau ; Evan Stefanek ; Sadaf Samimi Gharaie 等
期刊名称：iScience
印刷版ISSN：2589-0042
出版年度：2022
卷号：25
期号：5
页码：1-23
DOI：10.1016/j.isci.2022.104251
语种：English
出版社：Elsevier
摘要：SummaryCharacterizing the mechanical properties of engineered tissue constructs provides powerful insight into the function of engineered tissues for their desired application. Current methods of mechanical characterization of soft hydrogels used in tissue engineering are often destructive and ignore the effect of 3D bioprinting on the overall mechanical properties of a whole tissue construct. This work reports on using a non-destructive method of viscoelastic analysis to demonstrate the influence of bioprinting strategy on mechanical properties of hydrogel tissue scaffolds. Structure-function relationships are developed for common 3D bioprinting parameters such as printed fiber size, printed scaffold pattern, and bioink formulation. Further studies include mechanical properties analysis during degradation, real-time monitoring of crosslinking, mechanical characterization of multi-material scaffolds, and monitoring the effect of encapsulated cell growth on the mechanical strength of 3D bioprinted scaffolds. We envision this method of characterization opening a new wave of understanding and strategy in tissue engineering.Graphical abstractDisplay OmittedHighlights•3D Printed hydrogel scaffolds can be gently vibrated to evaluate mechanics•This method can evaluate complex multi-material scaffold mechanics•The method can evaluate the temporal evolution of cell-laden bioprinted constructs•Complementary insight to traditional mechanical analysis (rheometry, etc.) is gainedTissue Engineering; Materials science; Biomaterials