文章基本信息

标题：Trans-omics analysis of insulin action reveals a cell growth subnetwork which co-regulates anabolic processes
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作者：Akira Terakawa ; Yanhui Hu ; Toshiya Kokaji 等
期刊名称：iScience
印刷版ISSN：2589-0042
出版年度：2022
卷号：25
期号：5
页码：1-27
DOI：10.1016/j.isci.2022.104231
语种：English
出版社：Elsevier
摘要：SummaryInsulin signaling promotes anabolic metabolism to regulate cell growth through multi-omic interactions. To obtain a comprehensive view of the cellular responses to insulin, we constructed a trans-omic network of insulin action inDrosophilacells that involves the integration of multi-omic data sets. In this network, 14 transcription factors, including Myc, coordinately upregulate the gene expression of anabolic processes such as nucleotide synthesis, transcription, and translation, consistent with decreases in metabolites such as nucleotide triphosphates and proteinogenic amino acids required for transcription and translation. Next, as cell growth is required for cell proliferation and insulin can stimulate proliferation in a context-dependent manner, we integrated the trans-omic network with results from a CRISPR functional screen for cell proliferation. This analysis validates the role of a Myc-mediated subnetwork that coordinates the activation of genes involved in anabolic processes required for cell growth.Graphical abstractDisplay OmittedHighlights•Atrans-omic network of insulin action inDrosophilacells was constructed•Insulin co-regulates various anabolic processes in a time-dependent manner•Thetrans-omic network and a CRISPR screen for cell proliferation were integrated•A Myc-mediated subnetwork promoting anabolic processes is required for cell growthSystems biology; In silico biology; Omics