文章基本信息

标题：A human cornea-on-a-chip for the study of epithelial wound healing by extracellular vesicles
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作者：Zitong Yu ; Rui Hao ; Jing Du 等
期刊名称：iScience
印刷版ISSN：2589-0042
出版年度：2022
卷号：25
期号：5
页码：1-15
DOI：10.1016/j.isci.2022.104200
语种：English
出版社：Elsevier
摘要：SummaryOrgans-on-chips are microfluidic devices for cell culturing to simulate tissue-level or organ-level physiology and recapitulate their microenvironment, providing new and significant solutions other than traditional animal tests.In vitrotesting platforms for ocular biological studies have been increasingly used in preclinical efficacy and toxicity prediction. Here, we developed a microfluidic platform consisting of human corneal cells and porous membrane, replicating the multi-scale structural organization and biological phenotype. We verified the fully integrated human cornea’s barrier effects on the chip. Moreover, we found that extracellular vesicles derived from bone marrow-derived mesenchymal stem cells can significantly accelerate the mild corneal epithelial wound healing, and the decreased expression of matrix metallopeptidase-2 protein indicated that this method effectively inhibits corneal inflammation and angiogenesis. This work improves our ability to simulate the interaction between the human cornea and the external worldin vitroand contributes to the future development of new screening platforms for biopharmaceuticals.Graphical abstractDisplay OmittedHighlights•Combined human corneal cells and microfluidics to mimic ocular surfacein vitro•Integrated human cornea’s barrier effects on the microfluidic platform•In vitromodel of mild corneal epithelial wound to test cell-free therapeutics•Extracellular vesicles accelerate corneal epithelial wound healingBiological sciences; Bioengineering; Tissue engineering; Biotechnology; Cell biology