文章基本信息

标题：Lipid bilayer-assisted dynamic self-assembly of hexagonal DNA origami blocks into monolayer crystalline structures with designed geometries
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作者：Yuki Suzuki ; Ibuki Kawamata ; Kotaro Watanabe 等
期刊名称：iScience
印刷版ISSN：2589-0042
出版年度：2022
卷号：25
期号：5
页码：1-13
DOI：10.1016/j.isci.2022.104292
语种：English
出版社：Elsevier
摘要：SummaryThe DNA origami technique is used to construct custom-shaped nanostructures that can be used as components of two-dimensional crystalline structures with user-defined structural patterns. Here, we designed an Mg2+-responsive hexagonal 3D DNA origami block with self-shape-complementary ruggedness on the sides. Hexagonal DNA origami blocks were electrostatically adsorbed onto a fluidic lipid bilayer membrane surface to ensure lateral diffusion. A subsequent increase in the Mg2+concentration in the surrounding environment induced the self-assembly of the origami blocks into lattices with prescribed geometries based on a self-complementary shape fit. High-speed atomic force microscopy (HS-AFM) images revealed dynamic events involved in the self-assembly process, including edge reorganization, defect splitting, diffusion, and filling, which provide a glimpse into how the lattice structures are self-improved.Graphical abstractDisplay OmittedHighlights•Lipid bilayer-assisted self-assembly of 3D DNA origami blocks was achieved•Time-lapse AFM imaging of the self-assembly processes was performed•Different assembly patterns were achieved from a single DNA origami designSupramolecular materials; Materials science; Materials chemistry; Biomaterials;