文章基本信息

标题：The TORC1 phosphoproteome in C. elegans reveals roles in transcription and autophagy
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作者：Aileen K. Sewell ; Zachary C. Poss ; Christopher C. Ebmeier 等
期刊名称：iScience
印刷版ISSN：2589-0042
出版年度：2022
卷号：25
期号：5
页码：1-20
DOI：10.1016/j.isci.2022.104186
语种：English
出版社：Elsevier
摘要：SummaryThe protein kinase complex target of rapamycin complex 1 (TORC1) is a critical mediator of nutrient sensing that has been widely studied in cultured cells and yeast, yet our understanding of the regulatory activities of TORC1 in the context of a whole, multi-cellular organism is still very limited. UsingCaenorhabditis elegans, we analyzed the DAF-15/Raptor-dependent phosphoproteome by quantitative mass spectrometry and characterized direct kinase targets byin vitrokinase assays. Here, we show new targets of TORC1 that indicate previously unknown regulation of transcription and autophagy. Our results further show that DAF-15/Raptor is differentially expressed during postembryonic development, suggesting a dynamic role for TORC1 signaling throughout the life span. This study provides a comprehensive view of the TORC1 phosphoproteome, reveals more than 100 DAF-15/Raptor-dependent phosphosites that reflect the complex function of TORC1 in a whole, multi-cellular organism, and serves as a rich resource to the field.Graphical abstractDisplay OmittedHighlights•Detailed, unbiased analysis of theCaenorhabditis elegansTORC1 phosphoproteome•Characterization of DAF-15 expression and localization•TORC1 direct targets with roles in transcription machinery and autophagy•Unique technical approach and datasets provide rich resource to the fieldCell biology; Functional aspects of cell biology; Developmental biology; Omics; Proteomics