文章基本信息

标题：Lifetime of glass nanopores in a PDMS chip for single-molecule sensing
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作者：Mohammed F. Alawami ; Filip Bošković ; Jinbo Zhu 等
期刊名称：iScience
印刷版ISSN：2589-0042
出版年度：2022
卷号：25
期号：5
页码：1-14
DOI：10.1016/j.isci.2022.104191
语种：English
出版社：Elsevier
摘要：SummaryNanopore sensing is an emerging technology that has many biosensing applications ranging from DNA sequencing using biological pores to biomolecular analysis using solid-state pores. Solid-state nanopores that are more stable are an attractive choice for biosensing applications. Still, biomolecule interactions with the nanopore surface reduce nanopore stability and increase usage costs. In this study, we investigated the biosensing capability for 102 quartz glass nanopores with a diameter of 11–18 nm that were fabricated using laser-assisted capillary pulling. Nanopores were assembled into multiple microfluidic chips that were repeatedly used for up to 19 weeks. We find that using vacuum storage combined with minimal washing steps improved the number of use cycles for nanopores. The single-molecule biosensing capability over repeated use cycles was demonstrated by quantitative analysis of a DNA carrier designed for detection of short single-stranded DNA oligonucleotides.Graphical abstractDisplay OmittedHighlights•Maintaining low noise and linear current-voltage relation is crucial for biosensing•Multiple nanopores embedded in a chip can make more than 90 different measurements•Nanopores can be reused weekly over 19 weeks•Nanopore failure can be attributed to contaminant accumulation and dissolutionBiophysical chemistry; Biotechnology; Nanotechnology; Physical chemistry