文章基本信息

标题：Wastewater-Based Epidemiology for Community Monitoring of SARS-CoV-2: Progress and Challenges
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作者：Xing-Fang Li ; Xing-Fang Li ; Teresa Kumblathan 等
期刊名称：ACS Environmental Au
电子版ISSN：2694-2518
出版年度：2021
卷号：1
期号：1
页码：18-31
DOI：10.1021/acsenvironau.1c00015
语种：English
出版社：American Chemical Society
摘要：Wastewater-based epidemiology (WBE) is useful for the surveillance of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in communities, complementing clinical diagnostic testing of individuals. In this Review, we summarize recent progress and highlight remaining challenges in monitoring SARS-CoV-2 RNA in wastewater systems for community and environmental surveillance. Very low concentrations of viral particles and RNA present in the complicated wastewater and sewage sample matrix require efficient sample processing and sensitive detection. We discuss advantages and limitations of available methods for wastewater sample processing, including collection, separation, enrichment, RNA extraction, and purification. Efficient extraction of the viral RNA and removal of interfering sample matrices are critical to the subsequent reverse transcription-quantitative polymerase chain reaction (RT-qPCR) for sensitive detection of SARS-CoV-2 in wastewater. We emphasize the importance of implementing appropriate controls and method validation, which include the use of surrogate viruses for assessing extraction efficiency and normalization against measurable chemical and biological components in wastewater. Critical analysis of the published studies reveals imperative research needs for the development, validation, and standardization of robust and sensitive methods for quantitative detection of viral RNA and proteins in wastewater for WBE.
关键词：wastewater-based epidemiology;surveillance;SARS-CoV-2;Covid-19;RNA;RT-qPCR;controls