摘要:Biological processes run by microalgae are prospective but still little known methods of hydrogen production. A prerequisite for their increased advancement is the development of economically viable and efficient technologies. The study presented in this manuscript focused on determining the efficiency of biohydrogen production by P. subcordiformis using a culture medium prepared based on natural waters. The rate of P. subcordiformis biomass growth reached 317.6 ± 42.3 mgODM/dm3·d and ensured a biomass concentration of 3493 ± 465 mgODM/dm3. The percentage concentration of hydrogen in the biogas reached 63.2 ± 1.4%, and its production rate ranged from 0.53 ± 0.05 cm3/h to 0.70 ± 0.01 cm3/h.