文章基本信息

标题：Microbiome-by-ethanol interactions impact Drosophila melanogaster fitness, physiology, and behavior
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作者：James Angus Chandler ; Lina Victoria Innocent ; Daniel Jonathan Martinez 等
期刊名称：iScience
印刷版ISSN：2589-0042
出版年度：2022
卷号：25
期号：4
页码：1-23
DOI：10.1016/j.isci.2022.104000
语种：English
出版社：Elsevier
摘要：SummaryThe gut microbiota can affect how animals respond to ingested toxins, such as ethanol, which is prevalent in the diets of diverse animals and often leads to negative health outcomes in humans. Ethanol is a complex dietary factor because it acts as a toxin, behavioral manipulator, and nutritional source, with both direct effects on the host as well as indirect ones through the microbiome. Here, we developed a model for chronic, non-intoxicating ethanol ingestion in the adult fruit fly,Drosophila melanogaster, and paired this with the tractability of the fly gut microbiota, which can be experimentally removed. We linked numerous physiological, behavioral, and transcriptional variables to fly fitness, including a combination of intestinal barrier integrity, stored triglyceride levels, feeding behavior, and the immunodeficiency pathway. Our results reveal a complex tradeoff between lifespan and fecundity that is microbiome-dependent and modulated by dietary ethanol and feeding behavior.Graphical abstractDisplay OmittedHighlights•Flies produce more eggs on a low ethanol diet despite ingesting fewer calories•This increased reproductive efficiency does not occur for germ-free flies•Ethanol and commensal bacteria trigger immunity in a non-additive way•Intestinal barrier integrity is higher in flies consuming a low ethanol dietBiological sciences; Genetics; Microbiology; Microbiome; Molecular biology; Physiology