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标题：Functional and structural modifications of influenza antibodies during pregnancy
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作者：Madeleine F. Jennewein ; Martina Kosikova ; Francesca J. Noelette 等
期刊名称：iScience
印刷版ISSN：2589-0042
出版年度：2022
卷号：25
期号：4
页码：1-22
DOI：10.1016/j.isci.2022.104088
语种：English
出版社：Elsevier
摘要：SummaryPregnancy represents a unique tolerogenic immune state which may alter susceptibility to infection and vaccine response. Here, we characterized humoral immunity to seasonal influenza vaccine strains in pregnant and non-pregnant women. Although serological responses to influenza remained largely intact during late pregnancy, distinct modifications were observed. Pregnant women had reduced hemagglutinin subtype-1 (H1)- IgG, IgG1, IgG2, and IgG3, hemagglutination inhibition, and group 1 and 2 stem IgG titers. Intriguingly, H1-specific avidity and FcγR1 binding increased, and influenza antibodies had distinct Fc and Fab glycans characterized by increased di-galactosylation and di-sialylation. H1-specific Fc-functionality (i.e. monocyte phagocytosis and complement deposition) was moderately reduced in pregnancy. Multivariate antibody analysis revealed two distinct populations (pregnant vs. non-pregnant) segregated by H1 FcγR1 binding, H1-IgG levels, and Fab and Fc glycosylation. Our results demonstrated a structural and functional modulation of influenza humoral immunity during pregnancy that was antigen-specific and consistent with reduced inflammation and efficient placental transportGraphical abstractDisplay OmittedHighlights•Pregnancy resulted in structural and functional modulation of influenza antibodies.•Antibodies had differential binding capacity, Fc/Fab glycosylation, and function.•Antibody glycans directed toward low inflammation and efficient placental transfer.•Multivariate analysis of immune markers segregated pregnant and non-pregnant women.Virology; Pregnancy