文章基本信息

标题：Spatial proteomics reveals subcellular reorganization in human keratinocytes exposed to UVA light
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作者：Hellen Paula Valerio ; Felipe Gustavo Ravagnani ; Angela Paola Yaya Candela 等
期刊名称：iScience
印刷版ISSN：2589-0042
出版年度：2022
卷号：25
期号：4
页码：1-27
DOI：10.1016/j.isci.2022.104093
语种：English
出版社：Elsevier
摘要：SummaryThe effects of UV light on the skin have been extensively investigated. However, systematic information about how the exposure to ultraviolet-A (UVA) light, the least energetic but the most abundant UV radiation reaching the Earth, shapes the subcellular organization of proteins is lacking. Using subcellular fractionation, mass-spectrometry-based proteomics, machine learning algorithms, immunofluorescence, and functional assays, we mapped the subcellular reorganization of the proteome of human keratinocytes in response to UVA light. Our workflow quantified and assigned subcellular localization for over 1,600 proteins, of which about 200 were found to redistribute upon UVA exposure. Reorganization of the proteome affected modulators of signaling pathways, cellular metabolism, and DNA damage response. Strikingly, mitochondria were identified as one of the main targets of UVA-induced stress. Further investigation demonstrated that UVA induces mitochondrial fragmentation, up-regulates redox-responsive proteins, and attenuates respiratory rates. These observations emphasize the role of this radiation as a potent metabolic stressor in the skin.Graphical abstractDisplay OmittedHighlights•This work provides a systematic mapping of protein dynamic events triggered by UVA•Mitochondria were identified as one of the main targets of UVA radiation•UVA induces mitochondrial fragmentation and attenuates respiratory rates•A single, low UVA exposure induces DNA damage signaling responseCell Biology, omics, proteomics