文章基本信息

标题：The Identification of Amino Acids by Interpretation of Titration Curves: An Undergraduate Experiment for Biochemistry
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作者：Cassidy M. Dobson ; Nathan S. Winter
期刊名称：World Journal of Chemical Education
印刷版ISSN：2375-1665
电子版ISSN：2375-1657
出版年度：2014
卷号：2
期号：4
页码：59-61
DOI：10.12691/wjce-2-4-3
语种：English
出版社：Science and Education Publishing
摘要：Undergraduate biochemistry students should have great familiarity with titration curves. These curves allow the prediction of protonation states, charges, and isoelectric points. Here we describe an experiment in which students identify four amino acids based on their titration behavior. Students make solutions of each unknown amino acid and monitor the change in pH upon adding aliquots of a strong base. They identify the amino acids based on the shapes of the curves. They annotate the plots with isoelectric points, pKas, buffering regions and the structures of the amino acids.
关键词：titration curve; amino acids; pH; biochemistry; pKa; isoelectric point