文章基本信息

标题：Purification and Characterization of Bowman-Birk Trypsin Inhibitor from Soybean
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作者：Chunmei Gu ; Xinxiu Song ; Linlin Zhao 等
期刊名称：Journal of Food and Nutrition Research
印刷版ISSN：2333-1119
电子版ISSN：2333-1240
出版年度：2014
卷号：2
期号：9
页码：546-550
DOI：10.12691/jfnr-2-9-3
语种：English
出版社：Science and Education Publishing
摘要：In this paper, crude extract of a Bowman-Birk trypsin inhibitor from soybean meal was firstly isolated by a combination of , thermal denaturation, isoelectric precipitation and acetone precipitation. Then this extract was purified by DE-52 ion exchange and affinity chromatography. The results showed that soybean Bowman-Birk trypsin inhibitor (SBBI) was purified to 50.07-fold with trypsin activity of 822.31 U·mg-1. The purified SBBI gave a single protein band in SDS-PAGE electrophoresis. The accurate molecular mass of this inhibitor was determined to be 8837.46Da by MALDI-TOF. N-terminal sequence showed high homology with other serine proteinase inhibitors belonging to the Leguminosae family.
关键词：soybean Bowman-Birk trypsin inhibitor; purification; characterization