文章基本信息

标题：Anti-Proliferative Effects in Human Breast Cancer MDA. MCF-7 Cells & Human Breast Epithelial MCF-10a Cells and Western Blot Analysis from Adlay (Coix Lacryma-Jobi L.) Varieties Phenolic Extracts
本地全文：下载
作者：Lifeng Wang ; Huihui Xie ; Yumei Wang 等
期刊名称：Journal of Food and Nutrition Research
印刷版ISSN：2333-1119
电子版ISSN：2333-1240
出版年度：2014
卷号：2
期号：11
页码：792-799
DOI：10.12691/jfnr-2-11-6
语种：English
出版社：Science and Education Publishing
摘要：Analyzed the anti-proliferative activities (EC₅₀values) and cytotoxicities (CC₅₀values) of adlay extracts with adlay (Coix lacryma-jobi L.) varieties grains, Guizhou Heigu (hard hull, black), Liaoning 5 (soft hull, black) and Longyi 1 (hard hull, brown) to be the materials, normal human MCF-10a breast epithelial cells, human MDA breast cancer cells and human MCF-7 breast cancer cells to be the research subjects. After being processed by free or bound phenolic extracts of adlay, detected the expression levels of proteins such as PCNA, p21 and CDK4, which were related to cell proliferation and cell cycle in cells, by using western-blot test to preliminary obtain the mechanism of inhibition of tumor cells proliferation of adlay extracts. The results showed that the adlay extracts by different varieties had obvious anti-proliferative effects to tumor cells, and adlay samples themselves had no cytotoxicity in the tested experimental concentration range. Besides, adlay extracts inhibited the proliferation of HepG2 cells by regulating the cell cycle mediated by DNA polymerase adjustment factors such as PCNA and p21.
关键词：adlay polyphenol; cytotoxicity; anti-proliferative activity; protein expression