文章基本信息

标题：Purification of an Antiproliferative Lectin from Erophaca Baetica (Leguminosae) Seeds
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作者：Cristina Megías ; Isabel Cortés-Giraldo ; Julio Girón-Calle 等
期刊名称：Journal of Food and Nutrition Research
印刷版ISSN：2333-1119
电子版ISSN：2333-1240
出版年度：2013
卷号：1
期号：5
页码：87-91
DOI：10.12691/jfnr-1-5-2
语种：English
出版社：Science and Education Publishing
摘要：A lectin has been purified from the seeds of Erophaca baetica, an endemic legume of the Mediterranean Region. The protein has been purified from an albumin extract by gel filtration chromatography, after realization that affinity chromatography using Sephadex G-50 did not retain any proteins. Characterization of this protein shows that it is a 60 kDa homodimeric glycoprotein with 235 mg sugars / g protein, and two 30 kDa subunits. Its amino acid composition is similar to those reported for the lectins of other related legumes such as Astragalus mongholicus. It agglutinates trypsinized erythrocytes, and inhibits proliferation of human leukemic THP-1 cells. Thus, this novel lectin may be of interest from a functional point of view due to its antiproliferative activity.
关键词：Erophaca baetica; lectin; antiproliferative; THP-1 cells; seed proteins; legumes