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标题：Artepillin C Suppresses Angiogenesis by Inhibiting Tube-Formation and Inducing Apoptosis of Endothelial Cells
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作者：Mok-Ryeon Ahn ; Sun Wook Kim ; Shigenori Kumazawa 等
期刊名称：Journal of Food and Nutrition Research
印刷版ISSN：2333-1119
电子版ISSN：2333-1240
出版年度：2013
卷号：1
期号：5
页码：92-96
DOI：10.12691/jfnr-1-5-3
语种：English
出版社：Science and Education Publishing
摘要：Artepillin C (ARC) is a major active ingredient of Brazilian propolis which is a resinous mixed compound collected by honeybeesfrom various plant sources. Previously, we reported that the ethanol extract of Brazilian propolis possesses antiangiogenic activity both in vitro and in vivo. However, the mechanism of angiogenesis inhibitionby purified ARC has not been well-clarified. In this study, we investigated the effects of ARC on tube-forming human umbilical vein endothelial cells (HUVECs). We found that inhibition of tube formation by ARC was accompanied by partial fragmentation of endothelial cells, indicating that it induced cell death. Western blotting revealed that ARC induced the reduction of vascular endothelial cadherinand platelet endothelial cell adhesion molecule-1. ARC also induced chromatin condensation and cell nuclear fragmentation, morphological markers of apoptosis, in tube-forming HUVECs. Furthermore, ARC suppressed phosphorylation of extracellular signal-regulated kinase 1/2, but upregulated phosphorylation of p38. It was also shown that ARC induced apoptosis via activation of proapototic signaling, activation of caspase-3 and cleavage of poly ADP-ribose polymerase and lamin A/C. In conclusion, ARC exerts its antiangiogenic effects through induction of endothelial apoptosis.
关键词：angiogenesis; apoptosis; artepillin C; endothelial cell; tube formation