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标题：Optimization and Production of α -Amylase from Halophilic Bacillus Species Isolated from Mangrove Soil Sources
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作者：M. Kanimozhi ; Midhusha Johny ; N. Gayathri 等
期刊名称：Journal of Applied & Environmental Microbiology
印刷版ISSN：2373-6747
电子版ISSN：2373-6712
出版年度：2014
卷号：2
期号：3
页码：70-73
DOI：10.12691/jaem-2-3-2
语种：English
出版社：Science and Education Publishing
摘要：In the present study, four bacterial isolates from a mangrove soil were screened for their ability to produce α-amylase using submerged fermentation. Bacillus MJK1, MJK2, MJK6 and MJK10 which were assigned to be Bacillus species proved to be the best α- amylase producer. Various effects of pH, temperature, incubation time, carbon source and salinity were checked. Different carbon supplements were used to enhance the enzyme production and the highest yield was obtained with 2% soluble starch as supplements. The presence of fructose, maltose, sucrose, glucose reduced the production of amylase. The optimum pH, temperature, and incubation period for amylase production by the isolate was found to be 8.0, 50°C and 72 hrs respectively. The production medium with increase in addition of NaCl, diminished the production of amylase. The presence of NaCl in the culture media promoted extracellular amylase even in the presence of 4% NaCl.
关键词：α- amylases; Bacillus; mangrove soil; rhizosphere